Impact of the Anthropogenisation on the Metal Bioaccumulation and Distribution in the Spiny-Cheek Crayfish (Orconectes limosus Raf.) from Lake Gop³o, Poland

Document Type: Original Research Paper

Authors

1 Department of Biochemistry and Toxicology of Environment, Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland

2 Department of Ecology, Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Kordeckiego Street 20, 85-225 Bydgoszcz, Poland

3 Department of Soil Science and Soil Protection, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bernardyñska Street 6, 85-029 Bydgoszcz, Poland

Abstract

This study was undertaken to determine the concentrations of metals in the muscle and exoskeleton
of the crayfish Orconectes limosus in order to compare them with other species of crayfish and crustaceans.
In addition, we analyzed the correlation between the concentrations of the same element in the exoskeleton and
the meat and different elements accumulated in the same tissue. Crayfish individuals were acquired in spring
(May 2012) from Lake Gop³o. Individuals with damaged claws were not analyzed. Due to the relatively low
amounts of meat that was obtained from the abdomen of individual crayfish, material from individuals with a
similar body length was combined; thus, there were ten samples of meat and the exoskeleton obtained on the
whole. The muscle samples (about 12 g) were freeze dried in a Finn-Aqua Lyovac GT2 freeze dryer (Finland).
Three replicates of each freeze-dried sample were acid digested with 1 ml of concentrated HNO3 and 1 cm3
H2O2. Metal analyses were performed using an inductively coupled plasma mass spectrometry (ICP-MS)
with a Perkin-Elmer Optima 8300 spectrometer. The analyses revealed that the mean metals concentrations
(mg·kg-1) in the meat of the crayfish and in the exoskeleton were in the following order (Zn (115.571) > Mn
(18.825) > Cu (17.226) > Ni (15.472) > Pb (3.535) > Cr (0.769) > Co (0.551) > Cd (0.315) > Hg (0.138)) and
(Mn (111.640) > Zn (11.355) > Ni (8.165) > Pb (6.695) > Co (0.595) > Cu (0.575) > Cd (0.379) > Cr (0.195)
> Hg (0.0168)), respectively.

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