Persian Gulf is a Bioresource of Potent L-Asparaginase Producing Bacteria: Isolation & Molecular Differentiating

Document Type: Original Research Paper

Authors

1 Department of Miocrobiology; Jahrom Branch; Islamic Azad University; Jahrom; Iran

2 Molecular Medicine Research Center (MMRC); Hormozgan University of Medical Science (HUMS); Bandar Abbas; Iran

3 Persian Gulf and Oman Sea Ecological Research Institute; Bandar Abbas; Iran

Abstract

L-asparaginase is a candidate enzyme for anti-neoplastic agent againstacute lymphoblastic
leukemia and also extensively use in the food industry for prevention of acrylamide formation. L-asparaginase is widely distributed among microorganisms. In this study, marine bacteria were isolated from Persian Gulf and screened for L-asparaginase activity. Production of L-asparaginase was carried out by using M9 medium. Among L-asparaginase producing strains, 12 potent strains were differentiated based on nucleotide sequences of 16S rDNA. 12 potent strains included 2 strains of Pseudomonas spp., 8 strains of Bacillus spp, one strain of Zobellella spp. and one strain of Oceanimonas spp. identified and consequently the sequences published in the NCBI databases under the specific accession numbers. This is the first report on L-asparaginase activity of Zobellella spp. from this region. The highest (1.6 IU/ mL) and also the lowest (0.20 IU/ mL) productivity of L-asparaginase enzyme were recorded for Pseudomonas sp. PG_01 and Bacillus sp. PG_13 respectively. This study revealed marine bacteria are potential source of L-asparaginase enzyme .Pseudomonas sp.PG_01 with high productivity can be used for production of L-asparaginase. Therefore, Persian Gulf can be considered as a potential bioresource for L-asparaginase producing strains which may be effectively used in the large scale production by related gene cloning in purpose of medical application and food industry in the future.

Keywords