University of Tehran/Springer International Journal of Environmental Research 1735-6865 4 3 2010 07 01 The Biological Monitoring of Urinary 1-hydroxypyrene by PAH Exposure Among Smokers 439 442 228 10.22059/ijer.2010.228 EN K.H. Lee Department of Preventive Medicine, Seoul National University College of Medicine and Institute for Environmental Medicine, SNUMRC, Seoul, S. Korea S.H. Byeon Department of Environmental Health, College of Health Science, Korea University, S. Korea Journal Article 2010 11 03 This study examines changes in the urinary 1-hydroxypyrene (1-OHP) concentration as a polycyclic aromatic hydrocarbon (PAH) metabolite related to smoking among eight volunteers. The study subjects consisted of 8 participants (2 smokers, 4 ex-smokers and 2 non-smokers; mean age 23, range 21 ~ 24 years old) in a smoking cessation program. Three urine samples were collected each week for 4 weeks from each participant to give a total of 96 urine samples. None of the participants smoked during the study period. Levels of urinary 1-OHP, as an internal dose marker of smoke containing PAHs, were measured by high performance liquid chromatography (HPLC) fluorescence detector. Urinary 1-OHP levels in the ex-smokers were decreased during the 4 weeks study period, due to ambient sources of PAH exposure. Thus, smoking cessation was only expected to result in reduction of urinary 1-OHP. Urinary 1-OHP levels were significantly correlated with the amounts of cigarettes smoked, as assessed by the questionnaire (Spearman’s correlation coefficient, r=0.23, P=0.03). This study suggested that urinary 1-OHP can be a potentially useful biomarker of smoke. https://ijer.ut.ac.ir/article_228_95527e93ee9db6c98632fadc20c39311.pdf