@article { author = {}, title = {Toxic Effect ofAluminum OxideNanoparticles on GreenMicro-Algae dunaliella salina}, journal = {International Journal of Environmental Research}, volume = {9}, number = {2}, pages = {585-594}, year = {2015}, publisher = {University of Tehran/Springer}, issn = {1735-6865}, eissn = {2008-2304}, doi = {10.22059/ijer.2015.933}, abstract = {Aluminum oxide nanoparticles are the most widely used nanoparticles in various industries.Theincreasing use of nanoparticles in the past two decades and their entry into the industrial and non-industrialwaste water necessitates the assessment of potential effects of these substances in aquatic ecosystems. OECDstandard method was applied to determine the toxicity of this substance. After performing the detection rangetesting, the cells of 7 treatments and 2 controls were counted every 24 hours for 72 hours in three replicates foreach concentration. After extraction, chlorophyll a and carotenoid were measured using spectrophotometry.Scanning electron microscopy (SEM) was used to image the exposure of the algae cells to nanoparticles. The72-hour levels of EC10, EC50, EC90, and NOEC, specific growth rate (μ), doubling time (G), and percentinhibition (I%) were also calculated. The obtained 72-hour levels were EC10=1.6610-3, EC50=0.162, EC90=15.31,and NOEC=16.2×10-2mg/L. The control and treatment algae had a significant difference in terms of cell densityand growth inhibition rate (p<0.05). Aluminum oxide nanoparticles had a significant impact on the shape andtopography of Dunaliella salina cells and resulted in their swelling and enlargement. A significant differenceexisted in chlorophyll a and carotenoid concentrations between the treatment and control groups and the levelsof carotenoid decreased following increase in concentration of treatments (p<0.05). Aluminum oxidenanoparticles have a significant toxic effect on Dunaliella salina. With increasing nanoparticles concentration,Dunaliella salina chlorophyll and carotenoid concentration reduced significantly (p<0.05).}, keywords = {nanoparticles,toxicity,Aluminum oxide,Dunaliella salina}, url = {https://ijer.ut.ac.ir/article_933.html}, eprint = {https://ijer.ut.ac.ir/article_933_98c4f4e09daf835f91cd518d3a10eb04.pdf} }